we are planning to procure mass detector for UHPLC, do any one using ACQUITY QDa. What are the aspect we need to see before purchasing MS for LC.
Hi,
We use QDa's in the lab for a few analysis involving peptides; I think it is important to share the application(s) you envision to run using mass spectrometric detection, as that will help considering what points to look for.
You could think of what compounds are you planning to use, how large are these and how easily do these ionize, what sensitivity are you looking for, and/or mass accuracy, what robustness do you expect, ease of use (cleaning or repairing, for example).
Eef Dirksen Thanks for sharing your opinion. We are using UHPLC with PDA detector for PAHs , BPA and its homologous analysis in environmental matrices. We also want mass data for these compounds as reviewers are not always happy with PDA alone. Thanks
You should consult with someone local who has professional experience in LC-MS (> 10 years) and is familiar with the exact types of samples YOU wish to analyze and the matrix they will be found in. Only when the sample type(s) have been identified and analysis goals described can an appropriate analytical technique (and detector be suggested). It is well worth putting the needed time into researching and answering these questions before you contact the vendors. Some types of samples can not be detected via MS (or have very poor response). MS is not a 'universal' method (just as UV/VIS , not Universal). Using multiple detectors in series (DAD & MS) adds orthogonal data which is useful (and why we use multiple detectors for method development).
*Once your applications and goals are clearly described, review available systems. If possible, request a formal demonstration from one or more instrument vendors using one or two of your actual samples. Allow their experts to run your samples and show you the operation of the system and software. Be sure to have in attendance someone from your business who has professional experience using an LC-MS system as well as one of the potential key operator/users of the proposed systems present.
Be sure to purchase professional training form the instrument vendor after the system has been installed (include it in your budget). The vendor can help get experienced users up-to-speed on the basics of the system very fast (vs trying to figure it out yourself). After you have used the system to analyze many samples (~ 3 months later), consider paying for one or two more days of training/consulting too. You will have more questions at this time and be able to learn more. I mention these things now as they should be included in any budget for instrumentation. Fancy "tools" are of little value unless you have the many years of professional training needed to use them well. Investing in "Training" is far more important then selecting a specific system. Knowledge takes more than just time to acquire. It takes the right people with the right skills and experience too. Learn from them.
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