Optimum molar concentration of sulfuric acid to stop color reaction in ELISA?

20 October 2019 0 9K Report

We use sulfuric acid or hydrochloric acid to stop color development reaction in ELISA when TMB and H2o2 is used as chromogen and substrate, but the color is not stable if only acid is used to stop the reaction, what additive to acid can be used to stabilize color for longer time, for example 30 minute after adding acid

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