One step growth curve

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Anyone could tell me how to detect the protein cleavage product by SDS-PAGE?

i am currently transfecting a plasmid that express a protease, and this protease predicted to cleavage my target protein X by bio information prediction. In order to see my target protein X...

10 November 2011 2,183 6 View

Pdf file

anyone could send me the pdf file of Molecular Cell Biology (Lodish, Molecular Cell Biology) ???

10 November 2011 1,220 5 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

Isolation of Hydrogen Producing Bacteria?

any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...

13 July 2024 2,255 3 View

Hi. Can I get the list of publishers for microbiology-related short communications without publication fee (APC)?

I need to know which journals are exempt from publication fees (APC) in the area of microbiology

11 July 2024 6,346 2 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View

What are these aggregates in activated sludge flocs?

I've been working with activated sludge samples and have encountered a challenge in identifying certain structures/organisms that appear abundantly within the flocs during optical microscopy...

23 June 2024 9,584 1 View

Pseudomonas aeruginosa colonies are too transparent: any tips on counting?

Hi everyone. I need to do a lot of colony counting tomorrow and when I was doing it today I’ve noticed that most of the colonies are too transparent - I have to shine bright light on them all the...

12 June 2024 711 3 View