How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

How to show a protein is being dimerized in SDS-PAGE gel?

A protein dimerizes through CTD. I have expressed CTD of protein with two different tags. Now, I want to show that these CTD of protein dimerize in a pull-down assay. But my doubt is that if the...

29 January 2021 7,529 4 View

How to dissolve Scotch tape to collect the Au particles [analogous to the mechanical exfoliation of graphene]?

Hi everyone, I'm a mechanical person and now may need some help from chemistry experts. My question should be as easy as pie for the chemistry person. I prepared some Au particles in nanoscale...

21 January 2021 2,426 3 View

How commonly do you encounter Rummination Syndrome in your GI practice?

Do diaphragmatic breathing and speech therapy actually help? How often did you require multidisciplinary team approach?

17 January 2021 5,998 0 View

Is it correct to include variables as a group and as individual component in multivariable cox or logistic regression?

For example : If I am conducting the effect of metabolic syndrome on short term mortality of disease xy, can I enter "metabolic syndrome" as one variable and its components (diabetes,...

11 January 2021 6,348 2 View

It does not appear logistically possible to vaccinate everyone for COVID. So what's the point?

Public health authorities claim that no one is safe from COVID-19 until every one is safe. This has translated into a goal to vaccinate everyone who can be vaccinated. Logistically, however, it...

06 January 2021 4,269 12 View

Any one can tell me primer sequences used for q PCR to quantify MERS-CoV related coronavirus?

MERS-CoV primers

31 December 2020 2,389 2 View

How can I explain the rats' behavior in the open field test, if the induced rats stayed in the center area without movement?

I have worked on an animal model to induce fibromyalgia syndrome in rats when I tried to express data of open field test, I found that normal rats had moved fast after putting them in the central...

31 December 2020 7,736 3 View

Does anyone have any unreported cases of the Barakat (HDR) syndrome?

Barakat (HDR) syndrome is characterized by the triad of Hypoparathyroidism, sensorineural Deafness and Renal disease. In 2018 I published a paper entitled "Barakat AJ, Raygada M, Rennert OM....

29 December 2020 8,647 2 View