One of my scholar is doing research in early detection of down syndrome. Can any one suggest where we get down syndrome dataset for testing?

More Rajesh Ramachandran's questions See All

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

On what basis you choose Mass spec %Cov(95) cutoff score to validate a protein through CoIP?

I am working on centrosomal proteins. I overexpressed my POI and then I did SNAP capture pulldown followed by mass spec and I got approximately 700 protein hits. I am trying to validate specific...

03 July 2024 5,701 0 View

Is the wash in my IP the problem or something else?

The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....

01 July 2024 3,183 0 View

How can I better show my data?

I am validating the expression of several genes by qPCR to confirm transcriptome analysis. After many inconclusive runs, I realised my housekeeping gene (GAPDH) was downregulated in my...

27 June 2024 7,318 3 View

Nutrition map: What does it mean for you?

Unluckily, just similar to an expensive car, your brain can be damaged if you ingest anything other than premium fuel.

23 June 2024 2,287 7 View

How to optimize my MS2-tagged RNA pulldown in mammalian cells?

I am trying to do the affinity purification of MS2-tagged mRNA by capturing it on GST-MCP (MS2 coat protein) immobilized on glutathione beads. I used two approaches: transfecting the construct...

12 June 2024 504 0 View

How does Post-Intensive Care Syndrome (PICS) impact the lives of ICU survivors in the long term?

Navigating the Post-ICU Journey: A Holistic Approach to Reco... How does Post-Intensive Care Syndrome (PICS) impact the lives of ICU survivors in the long term?

25 May 2024 4,161 1 View

Should it really matter if we use Tris in place of HEPES as a component of lysis buffer for his-tag pull downs using Ni-NTA beads?

For making a general mammalian cell lysis buffer, I have been using HEPES as a component. However, I came across a paper where they have used Tris in place of HEPES while the rest of the...

28 April 2024 6,120 2 View

PCOS ovarian morphology in hypohypo patient compaired to PCOS patients?

the ultrasonic difference of the ovarian morphology in PCOS, and Hypogonadotrophic hypogonadism oligomenorrhic patient and PCO ovarian morphology

22 April 2024 8,821 1 View

Which HA beads are the best for Co-IP (drosophila tissue)?

Hi all, I am using HA beads to do a Co-IP (using drosophila whole body), then do mass spec to detect the interacting partners that pulled down by HA. The one that I tried before was not good, it...

09 April 2024 2,374 0 View