For some time I have been under the suspicion that my bacterial cells are undergoing "autolysis". The cells are grown in minimal media with at least 1% carbohydrate supplement. It is worth mentioning that these cells (Geobacillus thermoglucosidasius) can sporulate.

I'm looking for an efficient protocol to detect and perhaps quantify nucleic acid in bacterial culture supernatant. Does anyone have suggestions? Thank you!

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