I have metabolite concentrations from mammalian cells, and also total protein concentration in each replicate. I do not have an internal standard.
Please could someone describe or provide a literature reference/software method etc. of the best statistical method to normalize metabolite concentrations to total protein?
Many thanks
Dear Bibek,
Without internal standard, it would be very difficult. Do you have any other QC/QA analyses? You can check this paper to see if it helps:
Smart, K. F., Aggio, R. B., Van Houtte, J. R. & Villas-Bôas, S. G. 2010. Analytical platform for metabolome analysis of microbial cells using methyl chloroformate derivatization followed by gas chromatography–mass spectrometry. Nature Protocols, 5, 1709.
Please look at the following below links which may help you in your analysis:
https://academic.oup.com/bioinformatics/article/30/15/2155/2390365
https://www.scirp.org/pdf/ABB_2018081015055107.pdf
Thanks
hello，Bibek。 have you solved this problem? now I have IS and total protein. But I still confused how to normalize thie metabolomics data. should I use IS and protein both or other one of them, could you plesae help me? Thank you.
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