dear all, A Taqman system (primers + probe, HEX chanel) is giving off a lot of non-specific fluorescence at the end of the run in negative samples (6 Cq after the normal expected amplification in positive controls). If it is due to primer dimers, may a curve looking like an amplification curve be obtained? There is no contamination of the primers / mix! Can be the fluorophore HEX "leaky" ? If it is a primer/probe degradation why i don't always see it? Many Thanks for your help!