No Protein expression into E.coli.Looking for the suggestions?

29 December 2018 1 9K Report

I have cloned the gene of interest into the pet28a and pet 29a vector and transformed into the BL21, BL21DE3 and BL21DE3pLys. Still I am not able to find the expressed protein. can anyone suggest me the reason and what can i do the next?

Md. Golzar Hossain

You may try the followings

>Try with increased concentration of IPTG

>Different induction time and temperature

Finally if no expression, change the bacterial strain and plasmid constract.

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