need more informations
I am working with RAW 264.7 cells and my non-treated cells are showing high levels of pro-inflammatory cytokines. I have worked with them before in various experiments but this is the first time I...
25 August 2022 3,225 2 View
I need to know that if clinical trials are been conducted by a collaborative Institute (not at our facility) and they already have the "Clinical trial registry (CTRI) number", can we use that same...
24 February 2020 9,130 1 View
I have worked on a project that aims at testing the viability of latent semantic analysis in answering research questions in the field of translation studies. I sent the article to 4 or 5 journals...
01 February 2020 5,071 4 View
I am interested in tracing the way people talk about a specific topic on twitter. I have a set of hashtags, but I am not sure about the best tools to extract the related tweets.
31 December 2019 7,246 3 View
Historical data will be preferable, however cross-sectional data will also be helpful. The reason I prioritize historical data of the share of automation use in US manufacturing is due to the fact...
09 October 2019 2,771 1 View
In other words, I am going to run a single digestion and double by EcoRI and HindIII, as well as to the control ( undigested plasmid ) i have the kit from ThermoFisher which contains EcoRI...
05 June 2019 958 3 View
This strain have ability to produce gelatinase production or liquefaction or hydrolysis in gelatin containing media.
03 April 2019 3,979 0 View
Which are Scopus indexed computer science journals with less/free publication charges?
02 March 2019 1,092 2 View
Is there any rapid publication journals that are indexed by SCOPUS that cover the field of Bioinformatics?
11 December 2018 4,035 2 View
let A be an algebra and A1 the unitization of A, then multiplication algebra which is denoted by M(A) is algebra generated by identity operator and left multiplication and right multiplication...
11 December 2018 1,590 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?
28 July 2024 5,331 1 View
I need to install this program
25 July 2024 4,756 0 View
In general, when performing a Tafel analysis for corrosion resistance measurements, a line voltage scan is performed from say -250 to +250 mV vs. OCV at low scan speed around 0.125 mV/s. However,...
25 July 2024 7,982 3 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View
