Recently inherited a hypoxic incubator and am tasked with setting it up. The manual that came with the unit has minimal information regarding the size tank of N2 I should be getting. Hoping someone here has experience using an hypoxia incubator and can recommend the size of tank I should be purchasing and general maintenance. Our CO2 supply is central, but N2 supply needs to come from a tank. This is the Caron Hypoxic CO2 Incubator 170L fyi. Thanks!
For a Caron Hypoxic CO2 Incubator with a 170L capacity, you will need an appropriate nitrogen (N2) cylinder to maintain the desired hypoxic conditions. The size of the N2 tank will depend on the frequency of use and the desired oxygen levels. Use Standard Industrial Cylinder (e.g., K or T Cylinder)
If the incubator is used continuously or frequently, a larger tank or multiple tanks may be necessary to avoid frequent refills.Lower oxygen levels will require more nitrogen, thus depleting the tank faster.General Maintenance
Regular Monitoring: Check N2 levels regularly to ensure a continuous supply and avoid running out of gas.Periodically inspect connections and hoses for leaks.Ensure that regulators are functioning properly and replace them as needed.
Coordinate with your gas supplier to schedule regular refills based on your usage patterns.
Follow safety guidelines for handling and storing compressed gas cylinders.Setup Connect the N2 Cylinder,use appropriate fittings and regulators to connect the N2 cylinder to the incubator.Configure the incubator to the desired hypoxic conditions (e.g., 1-5% O2).Ensure that the oxygen sensors and other monitoring equipment are properly calibrated.
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