Hi

we also had some trouble with evaluating our fluorescence microscopy videos in matlab. Maybe VideoReader() is not compatible with the used file type/compression. This also depends on your system. We ended up with converting our videos to compressed *.avi files using ffmpeg.

Maybe check

https://www.mathworks.com/help/matlab/import_export/supported-video-file-formats.html

Hope this helps!

Sheher Bano Zaigham Matlab's error message "Error using VideoReader (line 103) The file does not exist" indicates that Matlab is unable to locate the video file that you are attempting to read in your code.

There might be several causes for this error:

1. The video file's file path is incorrect: Check that the file path supplied in the code is valid and that the video file is in the proper location.

2. The following video file is missing: Check that the video file is still present in the provided place and has not been removed by accident.

3. The following video file formats are not supported: Check if the

4. VideoReader function supports the video file format. Video file types supported are AVI,

The video file's codec is not supported: Check if the VideoReader function supports the video file's codec. Some codecs may not work with the function.

To resolve the issue, you can try the following solutions:

- Check to see that the video file is present and in the right location.

- Check to see if the VideoReader function supports the video file format.

- Check to see if the VideoReader function supports the video file's codec.

- Try running the function on a different Matlab version or computer to see whether the problem remains.

- You may also try alternative libraries for reading video files in Matlab, such as OpenCV, FFmpeg, and others.

If the issue continues, you should consult the Matlab manual and forum for further help debugging the VideoReader function.

Probably it's not a Matlab problem, but the compression codec, that was used to create that video, is missing from your computer while your colleague has it.

Thank you so much everyone for your valuable comments and guidance. I converted my video format into .avi and now I am able to run the code on my own system. Thank you all once again.