My lab recently recieved frozen blood samples in yellow-toppedthe serum tubes. Is it possible that we can extract serum from them?

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Banti Chakraborty

Kept the tube at room temperature (24 degree Celsius) until and unless the blood melted, then transfer 10ml of blood sample in centrifuge tube and centrifuge at 5000 RPM for 15mins. Then the serum and cellular part will be separated.

Furhan Iqbal

Banti Chakraborty Thanks for your reply. We centrifuged some samples on high speed for various duration but were not successful. will try 5000 RPM too.

Gabriel Betanzos-Cabrera

Hi,

The freezing breaks the erythrocytes and their components contaminant the serum, so your determinations will be affected. Try to separate the serum as soon as possible or put it in refrigeration temperature..