How to choose the appropriate stock model?

For the purpose of determining the optimum order quantity or the economic quantity of the order. There are many, many models used, on what basis we can choose the appropriate inventory model

04 June 2020 3,339 4 View

How can I analyse XRD data?

XRD operator provide me only peak lists: 2 theta values, FWHM and d-values. Could anyone suggest me the way of analysis these results?

11 December 2018 6,739 15 View

How seed and pod nanoparticles of moringa oleifera contribute to water purification?

after treatment water by seed and pod nanoparticles of moringa oleifera the water become purify so what reaction happened between them to get this result

11 December 2018 3,636 2 View

Please tell me how to calculate Crystal Size and intensity of peak using XRD Data?

I have the following data from XRD of my nanoparticles. kindly tell me how to calculate size/dia of these particles:

11 December 2018 759 7 View

How seed and pod nanoparticles of moringa oleifera contribute to water purification?

after treatment water by seed and pod nanoparticles of moringa oleifera the water become purify so what reaction happened between them to get this result

30 November 2018 539 2 View

How can I analyse XRD data?

XRD operator provide me only peak lists: 2 theta values, FWHM and d-values. Could anyone suggest me the way of analysis these results?

30 November 2018 3,570 12 View

How to interpret my FTIR spectrum? Is there any online software available to interpret the spectrum?

I want to know the exact functional groups for my FTIR Spectrum

10 November 2018 5,712 5 View

Why do the letter "Q" symbolize stream discharge?

Why do we usually use the letter "Q" to symbolize stream discharge? is it used in other sciences such as thermodynamics or fluid dynamics ... ? thank you.

25 October 2017 7,007 11 View

Thikness according to path in solid sheet model

21 March 2017 7,873 3 View

What is the simplest and easiest method used to determine the linear refractive index (n0) of solution (liquid)?

09 October 2016 2,452 6 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View