Murine C2C12 contractions

01 January 1970 1 8K Report

Hi all,

Currently I am working with murine C2C12 cells (MSc. Thesis). I have found some articles about the ability of C2C12 myotubes starting to contract spontaneously using some kind of electrical simulation. Is this also possible without any electrical simulation?

In short: I have some time and cells left. And I am looking for a (small) challenge. How can I let the C2C12 myotubes contract without any electrical simulation? Is it even possible?

Thank you your time!

Lotte

Gordon L Warren

I am definitely not an expert on this but if you allow the C2C12 cells to differentiate (i.e., switch to differentiation media) and mature, they will spontaneously contract. I have done calcium imaging on such cells and they light up like fireflies as they contract.

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