Dear colleagues,

We designed a transgenic cell line that gives us both a fluorescent and a luminescent output associated with different endpoints. Per se, we could culture and read out the fluo. signal on a black microtiter plate, lyse the cells, transfer the lysate to a white microtiter plate, and read out the lum. signal. However, I would like to increase the throughput by only using one of the mentioned plate types.

We are well aware of the caveats of using black or white plates regarding the fluo. or lum. measurement, as e.g. discussed here: https://se.promega.com/resources/pubhub/which-plates-to-choose-for-fluorescence-and-luminescence-measurements/

From your experience, which is the better plate type to use? Which one gives the better trade-off? Lum. is our primary signal, but as it is expected to have a better signal-to-noise ratio than the fluo. signal, I am inclined towards using the black plates.

Any kind of knowledge, experience, anecdotes, and trivia are much appreciated.

Thanks a lot.

Sebastian