mTOR1 is associated with myofibrilar synthesis such as myosin and actin (?). mTOR2 is regulator of actin cytoskeleton, so how they interact?
During exercise, would increased pH in the sarcoplasma lead to a different flux of H+ with lysosomes, as the pH in the cytosol will decrease? During exercise the pH can decrease due to protons...
01 February 2020 1,839 1 View
Would resistance training lead to a form of neurogenesis and changes of motor unit innervation of muscle cells? So RT can lead to alteration of the nervous system and creation of new branches or...
06 July 2018 3,844 0 View
Can we scientifically rate veganism? 1) It is claimed that DHA led to better functional brains. This would be important in the human developement. (is this correct?) 2) DHA is nowadays (what...
05 June 2018 2,408 0 View
Concurrent training. Would type IIx muscle fiber, not contain more myoglobin because it can be reached less efficient by oxygen because of fiber size? Would bigger muscle fibers that are hard to...
05 June 2018 7,008 0 View
Is sport science about individual holistic approach, or inter-individual reductionistic specialism? Should someone try to know as much as possible about everything related to the wide field of...
04 May 2018 3,917 5 View
In case of omega 3 supplementations and training effects, should there first be a ratio of 1:5 omega 3 to omega 6 before we can assess real effects? The ratio n-3 to n-6 is not balanced. This...
04 May 2018 8,842 1 View
is there literature about power lifting and weight lifting and the hamstring curl or knee flexor capabilities? Most they do squat, deadlift, goodmorning, straight leg deadlift, but it seems the...
04 May 2018 3,286 2 View
do we need white adipose tissue to a large extend on basis of hormons? Vitamins can be stored in IMTG, or in beige/brown adipose tissue. Energy we do not need with normal food stores. Isolation...
04 May 2018 3,299 2 View
Should the pectoralis major pars abdominal be trained with a different insertion position as the pectoralis major pars sternum and/or clavicula? The insertion is different for the three parts. So...
04 May 2018 2,708 0 View
How much fat tissue a person needs for normal hormonal functioning of adipokines such as leptin and others?
04 May 2018 1,548 0 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...
22 February 2021 6,937 3 View
Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...
18 February 2021 2,769 4 View
Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...
17 February 2021 2,383 3 View
I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...
17 February 2021 8,986 2 View
I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...
17 February 2021 7,802 2 View
I am doing research on the synthesis of paracetamol. I am preparing acetic anhydride solution with a few drops a sulfuric acid and wish to measure the pH of this solution. However my pH meter...
14 February 2021 8,699 3 View
I have chemically synthesized silver nanoparticle to combat microbes on wound site. I have used many methods of synthesis, but I found minimal field of silver NP product.I am getting whitish...
11 February 2021 4,964 13 View
Hi everyone! I extracted RNA from urine pellet using phenol-chloroform method. The yield of RNA was 160ng/uL. 3uL of RNA was used for cDNA synthesis in 20 uL reaction using Go-Script kit....
05 February 2021 6,587 4 View