Mouse tail base Injection and Finding Auxillary lymph nodes?

05 May 2018 4 4K Report

Hello.

Recently I'm working on simple immunologic studies.

However, I have no person who can teach me about immunology techniques I want to ask here two questions.

I read papers where the authors injected their vaccines into mice and they injected subcutaneously into the base of tail.

I also want to try this but I don't know where it is exactly.

When I inject at tail base, my syringe is located at tail side and needle is toward head. This way, when I put needle subcutaneously, the tip of the needle is quite apart from the very end of tail base. Is it okay for immunization?

Any kind of image would be super helpful to make me understood.

At the same time, I'm also struggling to remove lymph nodes from mice. I could isolate inguinal lymph nodes quite easily but it is not very simple to find axillary lymph nodes. Sometimes, I don't know if I am isolating axillary or brachial nodes. Do you have any know-how to find and remove axillary lymph nodes?

Thank you for your helps!

Nemanja Zdravkovic

Dear Kim,

Use this link to see how to find and remove axillary lymph nodes from mice.

https://www.jove.com/video/265/dissection-and-2-photon-imaging-of-peripheral-lymph-nodes-in-mice

Julie Joseph

Additional resources below.

1: SubQ injections:

Article Manual Restraint and Common Compound Administration Routes i...

http://www.procedureswithcare.org.uk/subcutaneous-injection-in-the-mouse/

2: Identifying LN: In the image linked below, you would see that axillary LN are on top while brachial are kind of hidden underneath the forelegs. Make sure you open the mouse up, place it on a styroform lid and position the carcass like in the image. I usually have to go through some skin to get to brachial.

http://www.informatics.jax.org/greenbook/figures/figure13-4.shtml

https://www.jove.com/video/3444/murine-superficial-lymph-node-surgery

https://www.jove.com/video/51803/isolation-of-murine-lymph-node-stromal-cells

Grant Gallagher

THis may help:

https://www.creighton.edu/fileadmin/user/medicine/images/TeachResearchPatient/Research/Flow_Cytometry_Core_Files/Protocols/Diagram_of_Mouse_Lymphoid_Tissues.pdf

good luck

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