Mouse primer (IL-8) ??

More Charith UB Wijerathne's questions See All

How can I run a single-point TDDFT?

How can I run a single-point TDDFT for 20 excitations? what is the command line should be in gaussian? Then I want generate NTOs from that, How can I do?

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How to calculate seasonal mean land surface temperature (day time) using satellite images?

i want to get seasonal mean land surface temperature for research about analyzing LULC and LSt.

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Comparing effect of a drug at Different temperatures with unpaired data?

Hi Everyone I have calculated the effect of a drug on the inactivation of a protein at different temperatures using unpaired experiments. The drug has a significant effect (p

11 January 2022 2,162 3 View

Any suggestions on the tripplet bands I observed at 72,56, and 45Kda for CSE/CTH protein in rat heart tissue?

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24 June 2020 5,009 3 View

How to Interpret Panel Regression Model with Low R squared and adjusted squared values?

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18 February 2020 3,625 3 View

Why inside out Single Channel Potassium currents are recorded with a positive holding potential?

Hi :) Im trying to start obtain on Single channel data on Potassium ion channels. So far I have worked with whole cell condition and I have no experience with single channel experiments. There is...

24 September 2017 2,563 7 View

Is it OK to Ground the patch Clamp Head-stage?

Hi all I was having an issue with my patch clamp rig. The membrane test was showing a small current (~100nA) even when the patch pipette was not touching the bath solution. Furthermore there was a...

19 April 2017 846 13 View

Permeability Calculation using Reversal Potential?

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11 December 2016 1,652 2 View

How to Calculate Time constant in BK channel Step Current recordings?

Hi I'm testing an effect of a drug on voltage gated potassium channel Slo3. I can see a clear change in current response to Step Pulses (shown in attached image). The voltage step protocol that...

12 July 2016 8,522 11 View

How do I Calculate/Plot a Conductance-Voltage Curve from Current-Voltage Data?

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06 December 2015 5,501 18 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

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31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

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30 July 2024 841 2 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

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25 July 2024 6,517 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

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23 July 2024 1,329 5 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

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20 July 2024 5,405 2 View

Where can I get PCR Primers for COI barcoding of birds?

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16 July 2024 118 2 View

Jasmine, Jia Ming Lee

Hi, according to what I know, mice don't express IL-8 gene, perhaps you should look up for the homologues?

For a good qPCR assay with proper primer design, our lab follows the MIQE guidelines, maybe you can check out the paper. Hope it helps.

Moftah H Alhagamhmad

As far as you know mice do not express this kind of chemokines "IL-8", what you can do is to measure one of the mouse analogues of human IL-8 like Monocyte Chemoattractant protein-1 ( MCP-1 ). Hope this help.......