I would like to know what kits can be used to extract mitochondrial DNA from fish liver and muscle tissue?
Do you want only the mtDNA (no nuclear DNA) or is it OK to get the nDNA as well? Any standard DNA extraction kit will give you both nDNA and mtDNA (e.g. Qiagen DNeasy kit).
Thank you for your reply! :) I would be interested in knowing if there is a commercial kit suited only for mtDNA extraction (without nDNA)?
There are kits that would at least enrich the mitochondrial DNA.
I think something like this would work for you https://www.thermofisher.com/order/catalog/product/89874#/89874
https://www.qiagen.com/se/products/discovery-and-translational-research/dna-rna-purification/dna-purification/plasmid-dna/qiagen-plasmid-kits/?clear=true#orderinginformation
I need it for journal's credibility. Thank you.
27 April 2024 6,101 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Are there any references that report the maximum isometric forces of shoulder muscles?
08 August 2024 6,060 0 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 648 0 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View