Greetings all,

I am trying to purify protein from squid ovary (Moisture content, 75%; protein content 19%). However, due to high amount of carbohydrates (8%) it hindering purification in the first step ammonium sulfate precipitation (unable to sediment the protein).

Therefore, I want to separate carbohydrate and proteins from my sample.

Mostly precipitation methods, using solvents like ethanol/acetone has been used to separate these component but functionality of one of the component is affected negatively.

Is there any other method used for separation of carbohydrates and proteins without affecting functionality of both components .

Please give me valuable comments/suggestions to sought out this problem.

Thanks and regards

Avtar