Greeting!
I am looking for a method to extract/isolate diterpenes i.e., cafestol and kahweol from coffee bean extract. Please suggest methods or protocol for it. Reference papers will be highly appreciated.
Kind regards,
Nikhil
This may be a starting point:
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000300017
Hi Nikhil
Please, Start with the Aqueous extract, Then with methanol 70% because coffee bean soluble .
thank you Nehal, if you don't mind, would you like to send protocol / procedure please?
Sorry Nikhil Bhandarkar; the most common method is the high performance liquid chromatography (HPLC), and particularly the hydrophilic liquid interaction chromatography,There are many Reference papers concerning this. Please revised them:
1.Lang et al;(2011).Molecular nutrition& food research 55(11):1613- 1623.
2.Liu et al;(2011).Determination of trigonelline in coffee powder and instant coffee by ultrasonic extraction and high performance liquid chromatography. Chinese journal of chromatography/ Zhongguo hua xue hui 29(11);1103- 1106.
3- Zhuo et al; (2010); Chinese journal of chromatography/ Zhongguo hua xue hui 28(4);379- 382.
Oigman, S. S., De Souza, R. O. M. A., Dos Santos Júnior, H. M., Hovell, A. M. C., Hamerski, L., & Rezende, C. M. (2012). Microwave-assisted methanolysis of green coffee oil. Food Chemistry, 134(2), 999–1004.
Novaes, F. J. M., Oigman, S. S., De Souza, R. O. M. A., Rezende, C. M., & De Aquino Neto, F. R. (2015). New approaches on the analyses of thermolabile coffee diterpenes by gas chromatography and its relationship with cup quality. Talanta, 139, 159–166.
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