How to obtain Contig sequence from BAM file?

I have use HISAT2 for alignment . Now i want to obtain contig sequence from from BAM file in galaxy server? I have used Samtools View. That is giving me some sequence but I am not sure whether it...

12 February 2021 3,262 4 View

Could you please share your experience in learning SPSS?

I would like to learn SPSS. Could you please guide me or share your experience if you feel it would help me to learn SPSS? Thank you very much indeed!

08 November 2020 7,357 6 View

How do I compare time domain solution with frequency domain?

I am solving EM wave scattering by PEC cylinder numerically and wanted to compare the scattering width. My result is in the time domain which is evolving with time but the analytical solution is...

11 October 2020 770 3 View

Is it possible to consider intermittency of renewable energy sources by using normally (Gaussian) distributed random numbers ?

Consider wind speed for wind power and solar radiance for solar power. Is it possible to consider intermittency of mentioned sources using normally ( Gaussian ) distributed random numbers in which...

29 January 2020 5,731 3 View

Could we download LANDSAT LST images from earth explorer site corrected atmospherically or the products corrected proposed are just for optical bands?

Could we download LANDSAT LST images from earth explorer site corrected atmospherically or the products corrected proposed are just for optical bands?

12 January 2020 9,036 3 View

How to prepare 1U of lyophilized powder of Bacillus stearothermophilus?

First of all, i have narrow knowledge about antidiabetic assay due to this is my first enzymatic test and i have no one to refer. I have a method but when i tried to google every each of the...

05 January 2020 4,629 3 View

How to compute unknown parameters in an equation from experimental data?

Is there a way to compute some unknown parameters in an equation using curve fitting using experimental data? I am upload a picture which contains the function and three unknown parameters and...

25 December 2019 2,184 7 View

From the Table attached, why Retentivity is decreased for Bi:ZnO 3% and for Bi:ZnO 5%, Coercivity is decreased but Ms is increasing linearly for all ?

Hi all, referring to the table attached, it is seen that when ZnO NWs are doped with Bi at different concentrations, there is an increase in magnetization values initially. But we see by doping...

08 October 2019 3,938 3 View

How to make 500 unit/ml catalase solution using catalase protein from Sigma?

13 August 2019 7,931 3 View

No title

10 March 2019 8,910 11 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

Why do my polyphenol measurements fail?

In order to measure the polyphenols in broccoli, we prepared a reagent for measurement using the following procedure. 1. Extract the crushed plant with 70% methanol. 2. Mix 1 ml of the extract...

07 February 2021 845 4 View

How to calculate Total Phenolic Content and express it in mg GAE/kg?

26 January 2021 3,847 7 View

GC-Headspace (HS-GC) chromatography for Forensic toxicological and Food expertises?

Dear Colleagues! How correct and appropriate is it to conduct forensic toxicological and food examination of volatile substances (ethanol, methanol, propanol, ethyl glycol, etc.) in blood and...

24 January 2021 5,645 2 View

Gibbs and fuel cell?

20 January 2021 6,146 2 View

No title

16 January 2021 8,334 5 View

Methanol usage in determination of vitamin B2 in foods using HPLC?

After acid hydrolysis and incubation in the dark using Taka-diastase enzyme, 30% sulphuric acid was added to stop the enzymatic reaction. The solution was diluted to the mark and some of the...

16 January 2021 3,740 4 View

How can I analyze using NMR spectroscopy; my compound is only soluble in hot methanol?

Halo every one I have isolated a compound from the methanolic plant extract. The compound is UV positive at both 254 and 365 nm. It seems to be insoluble in most solvents like CDCl3, CD2Cl2,...

13 January 2021 2,267 3 View

What is the solubility of Magnesium trifluoromethanesulfonate in methanol?

I am trying to dissolve Magnesium triflate in methanol. I took 2.6 g of it in about 20 mL methanol. After thorough stirring, I can see suspended particles! Can anybody throw some light on its...

12 January 2021 6,481 2 View