Is it possible to use common biochemical methods (chromatography, etc.) to determine the existence of metabolites naturally produced by microbiota in stools (i.e. acetate, butyrate)? We have no MS or NMR so that we're looking for unexpensive (and accessible) methods for these purposes.
Yes, it is possible. Perhaps the MALDI-TOF method is the best option, but the challenge is how to built the matrix
http://www.matrixscience.com/
http://www.ncbi.nlm.nih.gov/pubmed/25496753
I agree with Jose. You can also check out the following article:
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0070803
We used Gas Chromatography with a flame ionization detector (GC-FID) for the above linked paper (I am a co-author), but it was not coupled to MS and did not involve NMR. It is a fairly straightforward process if you have access to a GC-FID instrument.
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