Hi to all

But very interesting field

Sure it is. Thanks.

Yes, very interesting, but where are the "borders" respectivly the intersection to infectiology (in Germany a subtopic of internal medicine)? What are the areas of activity of Med. Microb. in other countries?

Interesting field

We are into diagnostic microbiology. We report on the patients' samples received at our laboratory. Provide diagnosis of infection. For example a fever can be dengue or malaria or typhoid or malaria or chikungunya. If the microbiologist provides diagnosis the physician will be in a better position to treat the cases with specific therapy

That's our main field too, but we are also busy with advisory serivce with reference to antibiotic therapy or with questions dealing with diagnosis of infection. We have a good connection to clinicians to our mutual benefit and I like this part of my job most!

hi Alrun, iam manal from Egypt ,microbiology department,my question is: how i can keep enterococci colonies after their culture ( on standard laboratory media such as blood agar - small beta-haemolytic colonies usually. Most species will also grow as pin-point lactose-fermenting colonies on MacConkey agar without crystal violet. They are aesculin positive), by any of the previous method mention for long time e.g more one year .And whtat is the media suitable for that ?my E- mail is http://[email protected]

Preserve in skimm milk

Panagea, preserve enterococci cultuer for more than one year in skimm milk ! please tell me more ,Did you try this method by yourself ? thanks.

Hi Manal, you can inoculate the enterococci in sterilisized milk in a little eppendorf cup and then freeze them with -18°C. When you need them for culture, let them grow warm in room temperature and then streak on normal agar medium.

I used this method by myself. Hope this is of help for you. Greetings from germany!

thanks, but what is the amount of the sterilized milk,type of milk ?also greetings from Egypt- mansoura city

We had conducted a short study. We cultured fecal samples of patients hospitalized and non-hospitalized patients. Cent percent of the hopsitalized patients on some or the other antibiotic showed presence of enterococci in the stool. But that was not true on patients treated on OPD basis.

Hi manal, i have tried and done work on enterococci pathogenic as well as non pathogenic enterococci, the very good medium i am regularly is using in my lab is URICHROM-II [ chromogenic medium] in gel form...

this is an Differential agar, but the enterococcus colinies on the agar is pin pointed small blur colour colonies...

you can reconfirm if required with microscopy, if you want to know wheather the enterococci is pathogenic or non pathogenic, you can perform a small and simple test it HRT test [ heat resistance test] if +ve then pathogenic...

the pathogenic enterococci is commonly is availabe in URINE samples, if you refer baley& scotch book of microbiology..

90% of Urine samples contain enterococci , after that only E.coli comes this study done in INDIA...

STORAGE:- skimmilked method OR Glecerol is an good methods to store the culture...

but you can use CRIYOvials, [storage vials for bacteria] very commponly availabe..

you want more information i can provide you the same on cryovials...

NOTE:- please do not isolate the enterococcus colonies from stool samples thata are non apthogenic and undergone mutations, so storage organisma will be mutated, so your pure culture will be an problem.

chromogenic agar called URICHROM will be a good agar to isolate..

Mobile number :- 09391123239 E.mail:- [email protected] OR [email protected]

thanks Navaneeth,please give me all information (in steps) : for example in our lab.i culture urine sample on CLED media then check colonies by gram film (always mixed bacteria, enterococci with others) so, i subculture these colonies on bile esculin ,then check colonies again by gram film,the result enterococci with others,then i do antibiotic sensetivity for vancomycin resistant enterococci ( VRE ), if resistant.iwil keep these colonies untill collect 20 one VRE for anther steps as API, E- test, so i am afraid from keeping not pure strain of enterococci ,and failure all my work. so please tell me your experaince to obtain pure strain of enterocci.And thanks again. please explain in detals the methods of storage as you mention : skimmilked method OR Glecerol as an good methods to store the culture.

Hi all although i think i am a bit late but better late than never as for u manal plz take care from mixed cultures it can spoil all ur results and take u to unending delma,so try to culture ur inoculum first in nutrient broth for alittle while then inoculate ur broth on the media selected according to ur need,after incubation try to take only one completely seprated colony from ur plate and streak it on a new one when u have, after incubation colonies which r morphologically alike then carry out ur api test.

thanks,Reham: write more about you e.g what is the name of your university? your reserch about enterococci?

Sorry manal for being so late but better late than never any way i am ein shams university i am an assistant researcher at the nuclear energy authority (national center for radiation research technology)-nasr city.

Hi manal..

you required any further help for enterococcus...

Hi Manal, If you have lyophilizer you can store the strains for very very long time.

I want to isolate some pentose(Xylose) fermenting yeast. what should be the source?