We have four strains of E coli :

C3-1

E2348 169

BW  25113

MG 25119

There are about 20 ul cell pellets from 1 ml anaerobic growths from each strain. We need to get more cells.

Should we grow them, anaerobically or aerobically, and in what media, minimal M-9. LB, or rich media?

Should we grow them all or just start with the K12 lab strain (MG) grown aerobically in LB?

Thank you very much.