How do you multiple impute item scores and perform a linear regression on factor scores?

I am developing a questionnaire and first performing an exploratory factor analysis. After I have the final factor structure, I plan on regressing the factor scores on some demographic covariates....

18 January 2021 883 8 View

How to remove dried cells/DNA off a nitex membrane for DNA extractions?

I'm working on developing a method to extract and isolate DNA from respiratory condensate or "blow" samples collected from beluga whales. In the field, we tried 2 different collection devices, a...

28 August 2018 4,866 2 View

Why are my SYBR green standard curve dilutions not amplifying and have flat curves?

Please help! Recently our SYBR Green qPCR reaction using our housekeeping gene, RPLP0, and all the same conditions stopped working one day to the next. We isolate RNA from tissue culture human...

03 August 2017 2,418 13 View

How to extract enough RNA from jellyfish polyps (Aurelia aurita) to monitor the expression variations of some genes (RT-PCR and qPCR)?

I would be very grateful if the community could give me some tips: as these organisms are very small, I wonder how many polyps do I have to pool for one RNA extraction or if one is enough? What is...

29 January 2017 4,605 2 View

Does anyone know what the average cell diameter of Panc1 cells is?

27 November 2016 5,872 3 View

In case of infected root samples, is the infection stopped by the Gus solution or by the ethanol washing bath (+ ref please :) )?

In my case, I'm working with entire root systems of Medicago truncatula infected by an oomycete.

18 October 2016 2,471 4 View

Why can I not digest or sequence my plasmid?

I amplified a plasmid and now I have an eluted stock from a maxi prep i did. I sent the plasmid out for sequencing and it came back as no priming. I tried a restriction digestion with 2 different...

13 August 2015 4,170 6 View

Any suggestion on the seeding density for primary monocytes in a 96-well?

I am doing a one-day assay with primary monocytes and seeding them at 3*10^5/well, as per a colleague recommendation. However I see others recommend much lower, but they seem to have different...

03 June 2015 6,422 2 View

How do I stimulate cell cultures with beta-estradiol?

I have been doing cell culture experiments that involve treating the cells with estradiol (E2). There are many papers published that state these cells are responsive to estrogen. The problem is...

15 May 2015 2,953 6 View

Can I use DataAssist or ExpressionSuite software for analysis if I acquired my qPCR data on the LightCycler 480?

As opposed to using Excel for analysis.

23 January 2015 5,201 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Why at farmer level Cow/Buffalo are not done artificial insemination just after 2 month for yearly calving?

Physiologically in cow, oestrous heat again goes on the peak after 2 months but locally this is not observed and AI is after two months but done after at least 6 months.

17 February 2021 685 4 View

Possible reasons for high bioavailability after I.M and S.C. routes...?

What should be the possible reasons if we obtained more than 100% bioavailability of antibiotics (marbofloxacin) after I.M. and S.C. routes of administration in cattle species.

16 February 2021 971 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View