Measuring AGEs, RAGE, CLM, CEL in sperm and testicular tissue?

More Stefan S Du Plessis's questions See All

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Which compound is getting oxidized during bulk electrolysis with TFAA?

The experiment is really simple. When I do a bulk electrolysis with 1% TFAA (ACN solution) to get dihydrogen, what is going to be oxidized? (working electrode graphit, counter electrode platinum,...

18 February 2021 4,430 1 View

Which non-coordinating solvent dissolves anhydrous Iron(II)chloride?

Hallo, i am looking for a non coordinating solvent that dissolves anhydrous FeCl2 for a synthesis in which i can not use solvents like thf, pyridin etc.. I already tried benzene (no solubility),...

11 February 2021 6,105 1 View

No title

Hi there! I'm very new to r and trying to run a multi-level meta analysis using pre-calculated effect sizes. I've attached the datafile below. The script I used as a first step to fit the model...

12 January 2021 8,214 2 View

How to extract within-group effects from a mixed effects model in R?

Hello, I am working with some longitudinal data resulted from a clinical trial. I have the interaction effect of time and group + random effect of Subject. Time variable consists of 6 timepoint...

29 November 2020 6,416 3 View

How can i define a correct baseline for reading the anodic (forward) peak current when oxygen reduction reaction is distorting my cyclic voltammogram?

Dear colleagues I have troubles to read out the correct anodic peak currents from my cyclic voltammogram since ORR is pushing down my baseline. Is there anything (besides substracting a proper...

23 November 2020 4,654 6 View

Changes in the spatial distribution of widespread mosses under climate change?

Are there any publications or own observations on how the spatial distribution of widespread moss species such as Pleurozium schreberi, Pseudoscleropodium purum or Hypnum cupressiforme changes...

12 November 2020 6,758 6 View

How can Mplus handle missing data in ESEM with rotation target?

Hello SEM Fans, I conducted an ESEM with target rotation using personality data. 1) Am i right that with rotation target, the calculation can only be made using listwise deletion? In this...

09 November 2020 8,972 3 View

Is there a database of photos with insect pollinators on flowers of plants?

I would like to know if there is a database of photos with insect pollinators on flowers. The insects must be located on flowers of plants. I am interested in pollinators encountered in Europe,...

04 November 2020 2,219 6 View

What program is best for the computer-assisted phonetic comparison of dialects?

What program is best for the computer-assisted phonetic comparison of dialects? We would like to compare several phonetically quite close dialects of a more or less well-documented language (with...

05 October 2020 5,336 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

Can I heat my ELISA at 37C?

Hello, I am conducting a sandwich-type ELISA. It is to see if small molecules can interfere with a protein-protein interaction. As this interaction would occur in the human body, may I heat...

31 January 2021 4,271 3 View

Translating two biomarkers to the same scale??

Hello researchers, I was wondering if anyone had experience in the following: I would like to translate two separate continuous biomarkers (A and B), that are mechanistically linked to each...

25 January 2021 2,107 3 View

How to dissolve Scotch tape to collect the Au particles [analogous to the mechanical exfoliation of graphene]?

Hi everyone, I'm a mechanical person and now may need some help from chemistry experts. My question should be as easy as pie for the chemistry person. I prepared some Au particles in nanoscale...

21 January 2021 2,426 3 View

How commonly do you encounter Rummination Syndrome in your GI practice?

Do diaphragmatic breathing and speech therapy actually help? How often did you require multidisciplinary team approach?

17 January 2021 5,998 0 View

Is it correct to include variables as a group and as individual component in multivariable cox or logistic regression?

For example : If I am conducting the effect of metabolic syndrome on short term mortality of disease xy, can I enter "metabolic syndrome" as one variable and its components (diabetes,...

11 January 2021 6,348 2 View

It does not appear logistically possible to vaccinate everyone for COVID. So what's the point?

Public health authorities claim that no one is safe from COVID-19 until every one is safe. This has translated into a goal to vaccinate everyone who can be vaccinated. Logistically, however, it...

06 January 2021 4,269 12 View

Steingrimur Stefansson

Hi Stefan, are you looking at diabetic rats? Do you see AGE fluorescence in testicular tissues?

Stefan S Du Plessis

Hi Steingrimur. We have a rat model of obesity and the animals are actually pre-diabetic (we did not induce diabetis with streptozotocin). At this stage we are just collecting tissue and have not started the analysis. I am still trying to establish which method is best (reliable and accurate). We are thinking of measuring RAGEs via both Immunohistochemistry and Western Blots. Any suggestions?