I am trying to process the wheat crop data collected using ASD for reflectance. However, I see two different options in ViewSpecPro software- Absolute reflectance and reflectance(transmittance). May I know the difference between them and what should be appropriate in studying variation in leaf reflectance among wheat genotypes due to disease and stress?
Hello,
Usually, one measures relative reflectance on the field and, when back to the office, calculates the absolute reflectance values. A nice step-by-step manual and Matlab code for this can be found in .
We have used this methodology at our paper .
Hope it helps!
Good work.
Hi, can you explain
why it is necessary to select the Relative Reflectance ?
And in which situation we can use the Absolute Reflectance ?
Thank you,
All the best,
Cécile.
Hi all,
The relative reflectance is used when you have the white reference (~100%) and the absolute one when you use the field spec without any standard.
For further information see below:
1. https://www.shimadzu.com/an/uv/support/fundamentals/reflectance_measurements.html
2. Attached file (search "absolute")
I hope I was helpful.
Cheers
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