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Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...
28 February 2021 364 3 View
Hello fellow researchers, I recently have a problem with an HPLC coupled to an Orbitrap mass analyzer. I usually use some protein mix to make sure the equipment is ready to separate and read...
09 February 2021 3,595 8 View
In my research related to monitoring the cow behavior with help of sensors I have a permanent and principal problem with the data for model validation. I tried to find some available open-source...
26 December 2020 8,197 2 View
Hello, For a project which studies how teens deal with social media and their interfaces, I'm interested in so-called "activity theory" (also know as cultural-historical activity theory). I know...
21 October 2020 2,364 4 View
Hi everyone! Does anybody know about a good website or place to get some guidance on Northern blotting? Also, advice on blotting the RNA ladder?
07 October 2020 739 3 View
I am trying to design a vector as compact as posible to test DNA transcriptional enhancer and/or repressor activity, I already have a compact bacbone (pICOz vector), minimal promoter, translation...
22 August 2020 1,083 1 View
Hi everybody! I need to amplify phage DNA so as to be able to produce restriction profiles using different restriction endonucleases. Can anyone recommend which primers should I use? I know random...
06 August 2020 1,194 3 View
My current research is about SPC and I'm interested in tuning strategies for these types of predictive control approaches. The cost function is on the photo and the tuning parameters are Np...
23 July 2020 1,176 1 View
On the role of aluminum in TWIP steels
22 July 2020 5,670 4 View
There are some theories, as Pelgrum and Law (2003) suggested that it is necessary to make a switch between the teacher and the student roles. I mean, the student should be also the active role in...
15 July 2020 5,169 15 View
Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...
03 March 2021 6,317 1 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Pls share your thoughts
01 March 2021 7,330 4 View
What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.
01 March 2021 8,374 4 View
Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...
24 February 2021 1,356 8 View
Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...
24 February 2021 7,379 4 View
Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...
23 February 2021 7,796 4 View
Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?
23 February 2021 3,840 3 View
Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...
22 February 2021 3,322 3 View
I'm currently working on my Dissertation and I'm trying to think of the best analyses that I should use for my study. I'm using three questionnaires for this and two different groups, 1st group...
22 February 2021 1,351 4 View