What are the appropriate markers to detect resting microglia? Studies have reported IBA-1 which is also extensively reported in M1 polarization. Some other studies have reported CD11b and CD45 as well but those are also found in activated microglia. So is there any certain marker to check resting microglial state?
Iba1 and Cd11b are present in both the resting and the activated phenotypes by will be upregulated upon activation. Tmem119 has been proposed as one of the resting state marker.
Hi Nasar Ullah Khan Niazi
Resting microglia are CD11bhi, CD45low. Also, IBA-1 is a protein abundantly expressed in microglia, both resting and activated. However, resting microglia have a characteristic ramified morphology with extended processes, which can be visualized using IBA-1 staining.
Please find the attached reference:
https://www.abcam.com/neuroscience/microglia-markers
Thank you,
Hi Nasar,
I would recommend P2Y12, this receptor is very shortly up-regulated after activation but then it is down-regulated in activated microglia.
Best regards,
Carsten
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