Could I use conventional PCR for the diagnosis of Marek’s disease in infected flocks?
DEAR .Dr. this link i suggest
https://fvl.vfu.cz/.../infectious-diseases.../cm_7-poultry.ppt
https://fvl.vfu.cz/.../infectious-diseases.../cm_7-poultry.ppt
GPCR tests have been developed for the diagnosis MD.Real-timequantitativePCR(qPCR)toquantity MDV genome copies hasalsobeen described(Abdul-Careem et al., 2006;Baigent et al.,2005; Islam et al.,2004).Inaddition , PCR tests that enabledifferentiation ofoncogenic and non-oncogenic strains of serotype 1 MDV, and of MDV vaccine strains of serotypes2 and 3 (Becker et al.,1992;Bumstead et al.,1997;Handberg et al.,2001;Silva, 1992; Zhu etal.,1992)have been described.
DEAR Dr.
This link may help you:
https://www.ncbi.nlm.nih.gov/pubmed/18645767
kind regards
https://www.researchgate.net/deref/https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F18645767ع
Dear Dr. Alaa this may help you
https://en.wikipedia.org/wiki/Marek%27s_disease
hi doctor see this link
https://en.wikipedia.org/wiki/Marek%27s_disease
www.ejmanager.com/mnstemps/98/98-1365191341.pdf
this link have PDF may be useful for you
Hi Dr.
www.oie.int/fileadmin/Home/eng/.../2.03.13_MAREK_DIS.pdf
this link will help you
Hi
Marek's disease is a Herpes virus infection of chickens, and rarely turkeys in close association with chickens, seen worldwide.
The disease has various manifestations: a) Neurological - Acute infiltration of the CNS and nerves resulting in 'floppy broiler syndrome' and transient paralysis, as well as more long-standing paralysis of legs or wings and eye lesions; b) Visceral - Tumours in heart, ovary, tests, muscles, lungs; c) Cutaneous - Tumours of feather follicles.
Diag.
History, clinical signs, distribution of lesions, age affected, histopathology. Differentiate from Lymphoid leukosis, botulism, deficiency of thiamine, deficiency of Ca/Phosphorus/Vitamin D, especially at the start of lay.
regards
Please find the attached file about
Marek'sDisease Virus, disease diagnosis
Regards
GPCR tests have been developed for the diagnosis MD.Real-timequantitativePCR(qPCR)toquantity MDV genome copies hasalsobeen described(Abdul-Careem et al., 2006;Baigent et al.,2005; Islam et al.,2004).Inaddition , PCR tests that enabledifferentiation ofoncogenic and non-oncogenic strains of serotype 1 MDV, and of MDV vaccine strains of serotypes2 and 3 (Becker et al.,1992;Bumstead et al.,1997;Handberg et al.,2001;Silva, 1992; Zhu etal.,1992)have been described.
Dear Dr. Alaa
The disease has various manifestations:
1. Cutaneous - Tumours of feather follicles.
2. Visceral - Tumours in heart, ovary, tests, muscles, lungs.
3. Neurological - Acute infiltration of the CNS and nerves resulting in 'floppy broiler syndrome' and transient paralysis, also more long-standing paralysis of legs or wings and eye lesions.
Diagnosis.
History, clinical signs, affected age, distribution of lesions, , histopathology. Must differentiated from Lymphoid leukosis, botulism, deficiency of thiamine, deficiency of Ca/Phosphorus/Vitamin D, especially at the first period of lay.
regards
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