Does anybody use Oxoid MSA agar plate as main medium for nasal screening for MRSA?
Do you have the problem that Gram negative bacteria grows after 24 hours +35'C +/- 2'C incubation period under aerobic condition.
Isn't the MSA media due to it's high salt content (7.5%), only Halophile and Staphylococcus sp. can growth on MSA? Some strains like Proteus mirabilis only very limited growth.
Why when I culture with Klebsiella sp., E. coli, and patient nasal swab sample, Gram-negative bacilli grows?
Any ideas? Or does anybody has any suggestion what media can be used for MRSA screening beside MSA and Mueller Hinton + 4% NaCl + Oxacillin ?
Thanks for answering my question.
I tried 2 difference batch of MSA, but this issue resolved with the company.
I uses MSA is to detect the Staphylococcus aureus colonies, then only proceed to antimicrobial sensitivity pattern (3ml peptone water, make it 0.50-0.60 McFarland, strike on 3 Mueller Hinton agar, and 13 diffierents type of AST disc place on it.) according to CLSI guideline.
Because I want to know the antimicrobial sensitivity pattern of MRSA.
Thank you.
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