Macrophage differentiation/polarization on uncoated glass coverslips

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Can you suggest a stable miRNA control in human endothelial cells (HUVEC) which is not influenced by cytokines?

We already tried RNU6-2 but failed .... any suggestions? Thank you!

08 September 2014 5,509 6 View

What is a good marker to distinguish between bladder urothelial cell and smooth muscle cells using PCR analysis?

We separated the urothelium by stripping and now want to show that there are no smooth muscle cells left in the urothelial cells preparation and vice versa. For smooth musle cells I will use...

03 April 2013 8,453 14 View

I am looking for a suitable immunoprecipitation antibody for TRAF6 in human cells. Any suggestions?

I would like to use protein G dynabeads.

09 October 2012 1,577 3 View

Fabrication of narrow glass capillary (inner diameter 200~500 nm)?

I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?

08 August 2024 8,915 0 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

Human macrophages cell surface markers?

I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...

29 July 2024 236 2 View

What is the right voltage sweep direction for characterization of HER kinetics?

In general, when performing a Tafel analysis for corrosion resistance measurements, a line voltage scan is performed from say -250 to +250 mV vs. OCV at low scan speed around 0.125 mV/s. However,...

25 July 2024 7,982 3 View

Detaching human-derived monocytes?

Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...

20 July 2024 7,922 2 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Differentiation THP1 cells into M2 macrophage with IL4 and IL13?

Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...

15 July 2024 5,153 2 View

FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View