Hi everyone, I was wondering if someone has had any experience purifying DNA at lower g or rpm values than indicated in a comercial minicolumn protocol. I'm using a Qiagen QIAamp DNA Mini Kit and I see that the different buffers go efficiently through the column at much lower g or rpm values than indicated in the datasheet (even in my tabletop microfuge that only goes up to 2000g). I wondered this mostly because the procotol indicates a g value that is not attainable with our current "biggest" centrifuge, so I thought I could try using lower g values, but I assume the yield might diminish somehow.

Thanks in advance !