What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How can I open a SXV4 file (SuperCROSS) in SPSS, R or STATA?

Hello everyone, I received some data from Statistics South Africa in an SXV4 format (SuperCROSS) and would like to open it in SPSS (or a similar programme if need be). I have tried opening it...

05 January 2021 2,463 4 View

Flow cytometric analysis of NBDG-uptake and intracellular staining possible?

Hello everyone, I am measuring glucose uptake into my cells by using NBDG and flow cytometry. Normally this works perfectly fine, but now I got some problems. When I fixed and permeabilised my...

25 October 2020 3,416 5 View

Does anyone know how I can see which nodes I chose for a specific file in NVIVO?

Hello! Does anyone know how I can see which nodes I chose for a specific file in NVIVO? I appreciate your answers, Katharina

02 October 2020 110 3 View

How can I analyse the modal ethnicity of a household and compare this to the ethnicity of the household head (SPSS)?

Hello everyone, I am working with a household survey and have both a person and a household file. I am using the household file for the main part of my analysis but want to explore the household...

21 July 2020 7,716 2 View

Who is working with Alzheimer's brain data?

Hi everyone, I am looking for a typical Alzheimer's brain for teaching purposes. I need a .nii file or similar. Of course the file should be showing a very typical brain and must be...

25 November 2019 7,917 3 View

Moderated mediation in PROCESS - Interaction not sig?

Hello, I'm computing moderated mediations using PROCESS for SPSS. The interaction effect for the moderation is not significant, however, the entire model for the moderated (!) mediation is...

16 December 2018 7,307 2 View

How to calculate molecular weight from m/z of oligosaccharides?

Hello, I did an enzymatic digest of alginate, an anionic polysaccharide from algae and performed MALDI-TOF. Now I have a chromatogram with several peaks but it do not know how to calculate the...

02 May 2018 4,165 3 View

Did anybody experience crystal formation in BrdU ELISA (Roche)?

We use the BrdU ELISA Kit from Roche a lot & never had any problems with it. Somehow, now we observe crystal formation (dark violett to black crystals) in the last steps of the assay... we did...

26 March 2017 3,668 2 View

I have very low luminescence units using Promega Dual Luciferase Assay, and I'm unsure whether I have low transfection efficiency or something else?

21 July 2016 1,935 5 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Could anyone recommend papers that focus on mixed-ethnicity Black-Caribbean/Caucasian adopted children?

Dear All, I have been looking for papers, or books, that focus on the issues surrounding adoption of mixed Black-Caribbean/Caucasian children. I am interested in literature that looks at any...

22 February 2021 8,466 2 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View