I am working on a droplet based digital biosensing project.
Assume I have some droplets (size 30-40um in diameter), each droplet contians aroun 10-20 Nano beads (100-300nm in diamter). The surface of beads are bonded with PEG-Beacon chain structures (click chemistry).
I am looking for a method that could aggregate these beads to one small zone or spot in these droplets.
I know magnatic beads was most frequently used method to achieve this propose, is there any other way to do it? I will have to use plastic or noble metal beads (Ag Au or Pt).
Dear Han Zhang, I thnk more details are needed. It may be done by: change of pH, addition of an anti-solvent, charge neutralisation, .... My Regards
Dear Han Zhang, I thnk more details are needed. It may be done by: change of pH, addition of an anti-solvent, charge neutralisation, .... My Regards
I mean everything is preadded to the droplet when generating them. After analytes bind and flouresent singal detected. I want to concentrate the singal by aggragate the NPs. I mean I can not add anything to the droplet after it is generated, so this condition may limit avaliable methods
Some nanoparticles are designed to aggregation by shaking or other stresses. Other stimulus are also possible. Please check the following documents. My Regards
https://doi.org/10.1021/ja056609n
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315612/
https://doi.org/10.1016/j.colcom.2016.02.002
https://doi.org/10.1021/la403963c
https://doi.org/10.1039/C8CS00787J
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040992/
Please check these articles:
Shaking-Induced Aggregation and Flotation in Immunoglobulin Dispersions: Differences between Water and Water–Ethanol Mixtures
doi: 10.1021/acsomega.0c01444
Stimuli-Responsive Gold Nanoparticles for Cancer Diagnosis and Therapy
doi: 10.3390/jfb7030019
CHARACTERIZATION OF NANOPARTICLE AGGREGATES WITH LIGHT SCATTERING TECHNIQUES
By Mariusz Woźniak
Best regards
- Badges
- Science topic
- Similar topics
- Chemistry
- Nanotechnology
- Nano