Hi, I am running a lipid extraction and quantification experiment from fish intestinal cells.

As extraction method I use the folch method, while for the quantification i use a Lipid Quantification Kit (Colorimetric), from cell biolabs (which uses Vanillin). After lipid extraction using the folch method I leave them to dry under the hood. When the chloroform has evaporated I resuspend the samples in DMSO. However, the lipids don't dissolve in DMSO. What is very suspicious about this is that when I initially did this experiment in another lab the extraction and quantification were working perfectly. I have tried any possible approach. The problem is not just related to the extra source of lipids, because even in the control, with just L15 and FBS, I still have some sort of pellet in the samples. Does someone have any idea about why this happens? Alternatively, does anyone know about a method of lipid quantification with samples suspended in chloroform?