Linearity in imaging immunofluorescence

01 January 1970 0 4K Report

Hej everyone,

If one were to access fluorescence intensity (per ROI for example) is there a proper way to analyse antibody/detection linearity (I'm thinking about a similar fashion as for western blotting)?

Secondly, is there any assumptions I can make about the underlying distribution (e.g. normality)of immunofluorescence output?

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