The role of pbotosynthesis in growth of in vitro-generated plants is controversial. Depend on hetrotrophic or autotrophic conditions the influence of photosynthesis on growth of the plants differs. I would be happy if somebody make it clear to me the influence of different wavelenghts using LED lights on photosynthesis of in vitro plants?
Autotrophy requires the shift to sugar free media in progressive steps. This treatment removes feedback ihnibition by photosynthesis end products, and has little to do with light source, if a fluence near 100 uEm-2s-1 is present. Reaction centers and antennae of photosynthesis have got a wide absorption spectrum with peaks in the blue and red region and only green bands are less efficient. The main use of leds is in red bands and the advantage is the ability to use pulsed light and long duty.; for a review you may take http://www.ncbi.nlm.nih.gov/pubmed/24642884
it is still worth while to look at the reb/blue ratio. some species very sensitive for light quality.
In lettuce it may activate some Tr factor,but the main use in vitro is about quantity and efficiency with much less heat
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