Ziptip vs C18 tip (Pierce) vs Stage tip (made in house) vs C18 spin tip/column (Pierce)
Dear Aiswarya,
I would recommend Stage Tips. The actual product, Empore C18 disks last a very long time and once you are equipped with the pick up, circular metal syringe end for coring the disks, you can handle many many samples for a very low cost. For large gel samples, I usually double the disk in a yellow pipette tip.
Best.
Hediye.
Dear Aiswarya,
I would recommend Stage Tips. The actual product, Empore C18 disks last a very long time and once you are equipped with the pick up, circular metal syringe end for coring the disks, you can handle many many samples for a very low cost. For large gel samples, I usually double the disk in a yellow pipette tip.
Best.
Hediye.
Dear Aiswarya,
In addition to stage tips, I would also recommend C18 Ziptip. It is a robust method, which offers the advantages including the speed, simplicity, lower consumption of organic solvent, and the potential to control the injection amount.
Best,
Chichi
Hello Aiswarya,
considering the cost there is a clear winner: stage tips (as Hediye mentioned allready)
If you have many samples to desalt i would also recommend the stage tipping procedure since you will be able to perform desalting with many samples in parallel in a centrifuge with easier handling in comparison to the ziptip procedure which is also taking longer. The efficiency of both procedures are comparable.
Another benefit of stage tips is the storage of your desalted peptides for up to monthes in a fridge (sometimes necessary to combine different experiments in one LC-MS run) before eluting them for LC-MS. Also the shipping of your peptide samples is much easier with stage tips without a need of cooling for a few days. I have received peptide samples on stage tips from another country, shipped in an envelope, and everything was working pretty fine.
Best, Murat
Always depends on the number of samples, viscosity and volume of the solution, the time you can spend on each sample etc. For just a few samples and working on a PostDoc time and money budget, go for Stage Tips; practice on a few model digests to make sure you get it right.
If you want to do 96+ samples per day in a reproducible fashion, the answer might be different, and even direct you to things like Oasis 96well plate formats.
Hi
One can also go for SCX clean up. Please go through the following article for better reference:
Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells
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