I want to use a 480-490 nm laser diode to excite cells for fluorescence (studying EGFP). Can somebody provide me with a suitable driver circuit that would be required to make the laser diode work?
Could you link the datasheet of the laser?
Required output power??
Hi, i am working on fluorescence microscopy..i am trying to build a small microscope to study fluorescence (eGFP) ..I would like to know if there is any limitation on the excitation time and...
03 April 2016 3,037 10 View
I have written an image analysis program in python (using opencv module)..now i want to use it to make an android app..Can somebody help me in this regard??
01 February 2016 1,602 4 View
I have an algorithm in the form of a python (.py) file..However, I want to run it on android phones in the form of an app..What is the simplest way to convert the .py file to android app? How to...
11 December 2015 8,898 2 View
I have a microfluidic device having five outlet channels, and two inlets. I am flowing two nutrients through the 2 inlets (eg: say YPD(c1) and SD(c2)). The two nutrients are mixed in different...
10 November 2015 5,321 3 View
Can somebody tell me how to calculate dead tinme of a photodetector, if I know the photodetector bandwidth? OR, what paprameters are required to calculate dead time? I found out from literature...
08 September 2015 5,964 0 View
I would like to generate optical delay of around 50 ns. But I dont want to increase the path length a lot, nor can I induce multiple reflections, since that would reduce the intensity of outcoming...
08 September 2015 794 17 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...
01 March 2021 6,474 3 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...
28 February 2021 2,116 1 View
Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...
28 February 2021 7,016 1 View
I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...
28 February 2021 4,890 3 View