Hello, I am trying to use laser capture to isolate entire subregions of the hippocampus in brain tissue (nonhuman primate.)
Some papers online say that sections should be cut between 8um and 12um. This seems extremely thin and I am wondering if this will provide too low a yield for RNA sequencing.
Does anyone have any experience cutting out entire structures or subregions with laser capture microdissection? What is the largest size you could cut tissue at? (20um, 40um, etc)
Jai Ghosh thank you very much for your reply. Why do you recommend thin sections? I was hoping to cut larger sections with the hope of yielding more/better quality RNA.
Tissue thickness is always a compromise between yield and contamination from other cells. I would also recommend something around 8-15µm.
However, if required for special applications, the MMI CellCut laser microdissection offers a software feature to cut thick tissue of more than 40µm (up to 80µm). This number is influenced by the sample itself, especially if the tissue is very wet.
Please let me know if you have any questions.
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