Hi,

I've been searching previous research successfully applied the metabolic RNA labeling (e.g. using 4-thio uridine) in bacteria for studying RNA dynamics but had no luck. Most of them focus on yeast or mammalian cells. Are bacteria known for the difficulty of metabolic RNA labeling? e.g. some of my speculations are that they are poor at taking up uridine from environment and/or do not synthesize UTP from scavange pathways quite well?

Would like to hear from people with related experience. Thanks!

Chun-Chen