I have a very thick date extract which cannot dissolve in a small amount of DMSO due to the presence of sugar
what techniques can be used to remove sugars or to get pure polyphenols or flavonoids?
Dear research
How can you be sure that this viscosity is due to sugars?
Most of the materials that make the extract thick and difficult to dissolve are phenolic substances with high molecular weights, as well as high turbines with molecular weights similar to rubber, where they are dried.
These impurities can be eliminated by purifying phenolic compounds and separating them using the acidification of the extract solution.
One moleartic acid can be added to the extract up to an pH=2
Then the phenols are withdrawn using chloroform several times using separating funnel
Best regards
Dr. Naser Jawad
University of Kufa Hanan Sa
I have published one article in the Journal of Packaging Technology and Research. But it is not in the list of JCR 2019 and also web science indexed journal. Can any one please explain. why the journal is not in the JCR list?Kindly help me to get the Thomson reuter impact factor of Journal of Packaging Technology and Research.
I'd try a couple of different methods-
- Ion exchange column to capture the flavanoids
- Polyamide resin
- Reverse phase such as C18
See: https://www.teledyneisco.com/en-us/liquidChromatography/Chromatography%20Documents/Posters/Purification%20of%20Phenolic%20Compounds%20Using%20a%20Combination%20of%20Flash%20and%20Preparative%20Chromatography.pdf
https://www.teledyneisco.com/en-us/liquidChromatography/Chromatography%20Documents/Posters/Purification%20of%20Phenolic%20Flavonoids%20with%20FC%20Poster.pdf
One more possibility is extraction with acidic water with dichloromethane or ethyl acetate. You may need to wash the aqueous phase several time with the organic solvent to get all the desired compounds. The acid keeps the phenolics as free acids.
Dear research
How can you be sure that this viscosity is due to sugars?
Most of the materials that make the extract thick and difficult to dissolve are phenolic substances with high molecular weights, as well as high turbines with molecular weights similar to rubber, where they are dried.
These impurities can be eliminated by purifying phenolic compounds and separating them using the acidification of the extract solution.
One moleartic acid can be added to the extract up to an pH=2
Then the phenols are withdrawn using chloroform several times using separating funnel
Best regards
Dr. Naser Jawad
University of Kufa Hanan Sa
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