JC-10 Mitochondrial Membrane Potential Kit: A Versatile Tool for Cellular Research

JC-10 is a fluorescent dye commonly used to measure the mitochondrial membrane potential (MMP) in living cells. It's a valuable tool for studying cellular processes like apoptosis, oxidative stress, and mitochondrial function.

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The JC-10 MMP assay is designed for live cell analysis and is sensitive to changes in mitochondrial membrane potential. The fixation and permeabilization steps in your flow cytometry protocol could disrupt the mitochondrial membrane potential, leading to inaccurate JC-10 results. Fixation generally causes JC-10 to lose its function, as it relies on intact mitochondrial membranes for accurate readings [1][2]. It is recommended to perform the JC-10 assay separately from your intracellular staining protocol. First, measure mitochondrial membrane potential in live cells using JC-10. Then, in a separate experiment, conduct surface and intracellular staining after fixation and permeabilization to ensure accurate assessments of both mitochondrial function and intracellular markers without interference.

Reference

[1]

Cossarizza, A., Baccarani-Contri, M., Kalashnikova, G., & Franceschi, C. (1993). A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1). Biochemical and Biophysical Research Communications, 197(1), 40-5.

[2]

Marcondes, N., Terra, S., Lasta, C. S., Hlavac, N., Dalmolin, M. L., Lacerda, L., Faulhaber, G., & González, F. (2018). Comparison of JC‐1 and MitoTracker probes for mitochondrial viability assessment in stored canine platelet concentrates: A flow cytometry study. Cytometry Part A, 95.