Hi,

Please See :

http://apsjournals.apsnet.org/action/showBook?doi=10.1094%2F9780890544969

PROTOCOL 01-02.1: Isolation of Phytophthora infestans from tomato leaves

Kelly Ivors

Laboratory Protocols for Phytophthora Species

https://doi.org/10.1094/9780890544969.01.02.1

ISBN: 978-0-89054-496-9

and see :

Mycologist

Volume 14, Issue 3, August 2000, Pages 137-139

Hi, 

in our research group "Fitotecnia Tropical" we used rye agar (RA)

(Difco, USA) for Phytophthora infestans sensu lato, and added different antibiotics, and these were incubated at 16°C with a photoperiod of 12 hours of light and 12 hours of darkness. 

Good Luck. 

Hi: Please find these useful papers:

http://apsjournals.apsnet.org/doi/pdf/10.1094/9780890544969.01.03.1

http://apsjournals.apsnet.org/doi/pdf/10.1094/9780890544969.01.02.1

http://www.sciencedirect.com/science/article/pii/S0269915X00800968

https://www.researchgate.net/publication/223064089_Isolation_techniques_and_cultural_media_for_Phytophthora_infestans_from_tomatoes

Article Isolation techniques and cultural media for Phytophthora inf...

here

here

Hi,

It’ s so easy to transfer inoculum (Mycelia) from tomato fruit to tuber disc of potato (previously sterilised with hypochlorite of sodium at 0.2% than washing with distilled water, incubation at 20°C for 4 days .

Concerning leaves, we can placed it in moisture roomat 20°C,  for sporulation.

After confirmation of the presence of no septate mycelia and sporangia of Phytophthora after isolation and microscopic observation . We  preleved developped mycelia into petri dishes containing Pea- agar medium (125 g of pea boiling in 1000ml of water + 15 to 20 g of agr), we can added antibiotic to eliminate bacteria contaminants. The incubation is in the same precited conditions. Good continuation.

Hello Priscillar

You can find useful references on attachment 1

https://www.ctahr.hawaii.edu/oc/freepubs/pdf/PD-45.pdf

2

https://www.researchgate.net/file.PostFileLoader.html?id=58890004ed99e108b6144cf3&assetKey=AS%3A454503322394625%401485373444601

3

http://biblioteka.ihar.edu.pl/show_pdf.php?src=pdf/04/02c53038.pdf&name=Michalska%20Anna%20Maria,%20Maria%20Pazio.pdf

4

https://www.rhs.org.uk/advice/profile?pid=217

5

https://hortsci.ashspublications.org/content/41/7/1635.full.pdf

6

https://fyi.uwex.edu/lincolncohort/files/2013/06/Organic-late-blight-control-publication.pdf

7

https://www.narcis.nl/publication/RecordID/oai%3Alibrary.wur.nl%3Awurpubs%2F421746

8

https://edepot.wur.nl/193819

9

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3464107/

10

https://vegetablemdonline.ppath.cornell.edu/factsheets/Potato_LateBlt.htm

11

https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-93-9-0947

12

https://link.springer.com/content/pdf/10.1007%2FBF01975422.pdf

13

https://phytopath.ca/wp-content/uploads/2015/03/DPVCC-Chapter-25-greenhouse-tomato.pdf

14

http://www.jmb.or.kr/journal/download.php?Filedir=../submission/Journal/023/&num=5639

d

https://www.plantphysiol.org/content/140/1/235.full

g

https://journals.iupui.edu/index.php/ias/article/download/13969/14054

s

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0109328

as

https://www.agriculturejournals.cz/publicFiles/35804.pdf

sa

https://scialert.net/fulltext/?doi=ajps.2008.284.290

n

https://www.pubs.ext.vt.edu/content/dam/pubs_ext_vt_edu/ANR/ANR-6/ANR-6_pdf.pdf