I am working on isolation of bioluminscent bacteria from fish and squids brought form local markets using ,different types of media,s such as photo bacterium medium,Boss media,marine agar,complete sea water media,but there is no bioluminscent bacteria,by checking agar plates in dark rooms,i have modified some conditions too like temperature,20C,22C,25C,35C,and glycerol and salts concentration from 1 to 4 %.Could someone advise me how to isolate bioluminscent bacteria from local market fish and squids.
Thanks
You may want to use selective media for Vibrio.
http://himedialabs.com/td/m820.pdf
Jay Y.S. Hodgson Thank you so much dear sir ,but i already used this media for my isolation but no positive results,spend 4 months on this but did not work for me.
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