How many restriction sites we could anchored to a primer?

I'm interesting to know that, 1. how many additional nucleotides can you add at the 5' site of the primer ? 2. How many restriction sequences can you add at the 5' site of the primer ? 3. when...

05 June 2018 9,494 6 View

Can RNA contaminate/ interfere plasmid sequencing?

06 July 2017 5,596 7 View

Can you help me to do ATPase assay using Glo Kinase assay kit(Promega)?

05 June 2016 9,359 3 View

If the protein contain a his tag, for western blot what sort of Ab is preferred?

My cloned gene contain His-C terminal tag. I would like to check my protein using western blot. So could you please advice me what sort of antibody could be use. I prefered to do the detection...

04 May 2016 8,330 4 View

In colony pcr, why some clones produce less intense bands while some are having very Intense bands in gel electrophoresis?

I use PCR to confirm the insert in my clones. Normally its getting positive or negative bands. But this time I got bands less intense than the positive with primer dimers. some clones only got...

03 April 2016 4,641 17 View

Are there any alternative start codon rather than ATG?

01 February 2016 6,777 6 View

Why 70% ethanol not 100% ?

31 December 2015 392 5 View

Can operon contain more than one promoter?

10 November 2015 1,709 1 View

What are the factors should consider when designing primers?

09 October 2015 7,558 2 View

What are the usages of N-terminal and C- terminal fusion tags?

pET-28a(+) vector contain few fusion tags. N-terminal His.Tag and N-terminal T7.Tag along with C-terminal His.Tag. What are the individual usage of each tags? If all tags were there with my insert...

09 October 2015 4,652 11 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View