Nilmini Kumari

13 Questions 40 Answers 0 Followers

Questions related from Nilmini Kumari

How many restriction sites we could anchored to a primer?

I'm interesting to know that, 1. how many additional nucleotides can you add at the 5' site of the primer ? 2. How many restriction sequences can you add at the 5' site of the primer ? 3. when...

06 June 2018 9,555 6 View

Can RNA contaminate/ interfere plasmid sequencing?

When preparing plasmid samples we do RNAse treatment to remove RNA from plasmid DNA. I would like to know; is it necessary for plasmid sequencing from sanger di deoxy method ? How RNA interfere...

07 July 2017 5,682 7 View

Is this SCI journal " International Journal of Scientific and Engineering Research "?

Please in for me details about this journal. It mentioned it is SCI journal listed in Thomsons Reuters journal list. But I couldn't find it! And please let me know the original web address of SCI...

03 March 2017 7,570 1 View

Can you help me to do ATPase assay using Glo Kinase assay kit(Promega)?

I would like to use above kinase assay kit to check the activity of ATPase in my expressed protein. But I couldn't find a person who has used it in my department. I would like if any body who has...

06 June 2016 9,418 3 View

If the protein contain a his tag, for western blot what sort of Ab is preferred?

My cloned gene contain His-C terminal tag. I would like to check my protein using western blot. So could you please advice me what sort of antibody could be use. I prefered to do the detection...

05 May 2016 8,400 4 View

In colony pcr, why some clones produce less intense bands while some are having very Intense bands in gel electrophoresis?

I use PCR to confirm the insert in my clones. Normally its getting positive or negative bands. But this time I got bands less intense than the positive with primer dimers. some clones only got...

04 April 2016 4,726 17 View

Are there any alternative start codon rather than ATG?

I have a bacterial gene sequence code for a protein but it's start position/ codon is TTG. Is it a mutation or consider as a alternative start codon?. I need to express this protein in a BL 21...

02 February 2016 6,841 6 View

Why 70% ethanol not 100% ?

In labs we are normally using 70% cleaning ethanol for sterilization. Can anybody know the theory behind it?

01 January 2016 603 5 View

Can operon contain more than one promoter?

In bacteria, protein coding genes are organized in an operon. If the operon contain three protein coding regions, My questions are; 1. Do all genes operate from one promoter? 2. Can individual...

11 November 2015 1,770 1 View

What are the factors should consider when designing primers?

I am going to design primers to amplify an operon from a gram positive bacteria in order to cloned in to a pGEMT vector. What are the steps I should follow?

10 October 2015 7,616 2 View

What are the usages of N-terminal and C- terminal fusion tags?

pET-28a(+) vector contain few fusion tags. N-terminal His.Tag and N-terminal T7.Tag along with C-terminal His.Tag. What are the individual usage of each tags? If all tags were there with my insert...

10 October 2015 4,731 11 View

How can I differentiate large size plasmids from genomic DNA ?

I have a wild type bacteria culture and want to check the presence of plasmids in it. I followed the normal plasmid isolation procedure and got a small pellet. In gel electrophoresis a lower...

12 December 2014 6,234 9 View

Identification of B. megaterium strain?

I am using 16S rRNA gene analysis for identification of bacteria. But for an organism show 100% 16S rDNA sequence (~1400 bp) homology to two species B. megaterium and B. ariyabattai according to...

01 January 1970 354 10 View