How can i extract the genomic material from micro-algal samples and microcystis strains?

I am working with some strains of cyanobacteria isolated from Zimbabwe's Manyame catchment - I want to extract their genomic material so that I may use it to confirm their identity. In my lab I...

09 October 2019 2,539 3 View

How can i start analysing data in SPSS Amos version 26?

I coded and captured my data in SPSS version 20, my challenge is how can I perform structural model analysis in Amos. How to start is where I am failing.

03 April 2019 6,443 1 View

Can i extract genomic DNA from cyanobacteria using phenol-chloroform?

can someone assist me with a protocol to extract genomic DNA from cyanobacteria using phenol-chloroform?

09 October 2017 8,031 4 View

May someone help me with a picture Bactrocera kandiensis?

Can someone help me with a colour picture of Bactrocera kandiensis?

03 April 2016 6,233 5 View

I am trying to use Stata 11 to perform ANOVA on my data set, can i have some assistance especially with the commands?

02 March 2016 1,311 8 View

Has anyone analysed the presence of Wolbachia strains in Bactrocera dorsalis and their effect ?

31 December 2015 2,002 0 View

Is this possible "ITS1 and ITS2 were aligned by eye (alignments available from the authors upon request)" - can someone help me?

11 December 2015 6,766 5 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View